นโยบายงานวิจัย /จรรยาบรรณนักวิจัย /ระดับคุณภาพบทความวิจัยตีพิมพ์ /ระดับคุณภาพผลงานวิชาการ /แหล่งทุน /ดาวน์โหลด /ฐานข้อมูลวิจัย /วิเคราะห์-สังเคราะห์งานวิจัย /ลิขสิทธิ์ /ข่าว


Bootstrap resampling technique to evaluate the reliability of the optimal liposome formulation skin permeability and stability response variables

Author

-

Sureewan Duangjit, Praneet Opanasopit, Theerasak Rojanarata, Jun Takayama, Kozo Takayama, Tanasait Ngawhirunpat


Journal

- Biological & Pharmaceutical Bulletin

Volume

- 37

Year

- 2014

Publication type

- Research article (Inter)

Page list

- 1543–1549

Abstract

   

A non-linear response surface method incorporating multivariate spline interpolation (RSM-S) is an useful technique for the optimization of pharmaceutical formulation., however, the direct reliability of the optimal formulation has to be evaluated. In this study, we demonstrated the feasibility of using the bootstrap (BS) resampling technique to evaluate the direct reliability of the optimal liposome formulation predicted by RSM-S. The formulation characteristics (Xn), including vesicle size (X1), size distribution (X2), zeta potential (X3), elasticity (X4), drug content (X5), entrapment efficiency (X6), release rate (X7) and the penetration enhancer factors (PE) as formulation factors (Zn), with the type of PE (Z1) and content of PE (Z2) were used as causal factors of the response surface analysis. The intended responses were the high skin permeability (flux, Y1) and the high stability formulation (drug remaining, Y2). Based on the dataset obtained, the simultaneous optimal solutions were estimated using RSM-S. Leave-one-out-cross-validation displayed the satisfying reliability of the optimal solution. Concurrently, similar BS optimal solutions were estimated from the BS dataset that was generated from the original dataset through BS resampling at frequencies of 250, 500, 750 and 1000. The analysis and simulation indicated that X4, X5 and Z2 were the prime factors affecting Y1 and Y2. These findings suggested that this approach could also be useful for evaluating the reliability of an optimal liposome formulation predicted by RSM-S and would be beneficial for the pharmaceutical development of liposome for transdermal drug delivery.


Keywords

   

Bootstrap, Response surface, Simultaneous optimal solution, Transdermal drug delivery