Factorial design of
essential oil extraction from Fagraea fragrans Roxb. flowers and
evaluation of its biological activities for perfumery and cosmetic applications
- Bancha Yingngam and Adelheid H. Brantner
- International Journal of Cosmetic Science (2013 Impact Factor 1.451)
- Research article (Inter)
To optimize the
extraction yields of essential oil from Fagraea fragrans Roxb. flowers
in hydro-distillation using a central composite design (CCD) and to evaluate
its biological activities for perfumery and cosmetic applications.
design was applied to study the influences of operational parameters [water to
flower weight (X1) and distillation time (X2)]
on the yields of essential oil (Y). Chemical compositions of the
essential oil extracted from the optimized condition were identified by gas
chromatography–mass spectrometry. Antioxidant activities of the essential oil
were determined against ABTS•+ and DPPH• radicals, and
the cytotoxic effects were assessed on human embryonic kidney (HEK293) cells by
the use of the MTT assay. Also, the aromatic properties of the essential oil
were evaluated by five healthy trained volunteers.
The best conditions to
obtain the maximum essential oil yield were 7.5 mL g−1 (X1)
and 215 min (X2). The experimental yield of the
essential oil (0.35 ± 0.02% v/w) was close to the value predicted by
a mathematical model (0.35 ± 0.01% v/w). 3-Octadecyne, Z,Z,Z-7,10,13-hexadecatrienal,
E-nerolidol, pentadecanal and linalool were the major constituents of
the essential oil. The essential oil showed moderate antioxidant capacities
with no toxic effects on HEK293 cells at 1–250 μg mL−1.
Also, the essential oil exhibited a very strong aroma and was classified to be
top- to middle-notes.
The results offer the
effectively operational conditions in the extraction of essential oil from F. fragrans
using hydro-distillation. The essential oil could be used as a natural
fragrance, having antioxidant activity with slight cytotoxicity, for perfumery
and cosmetic applications.